Rat HepatoPac Kits

Rat HepatoPac® Kits

Rat HepatoPac® kits are an ideal way to conduct all your DMPK, Predictive Toxicology & Mechanistic Toxicology applications. These kits come in a ready-to-use format of Rat HepatoPac; co-cultures micropatterned on industry-standard 24- and 96-well plates for streamlined, higher throughput in vitro assays. A variety of rat kits are available to cover all your application and size needs, and contain everything you need to conduct your assays. Begin dosing as early as 3 days after receiving the kit!

Rat Kit

Kits contain:

  • Rat HepatoPac Co-Cultures
  • Maintenance Media
  • Application-specific Media and additives
  • Instructions for culture maintenance and application-specific protocols
  • On-site customer training included

Satisfaction Guaranteed! Every kit is backed by Hepregen's performance guarantee and professional customer scientific support. If you are unable to produce desired results in your lab, Hepregen's scientists will run your compounds through our in-house pipeline.

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Validation

Rat HepatoPac in vitro co-cultures remain viable and highly functional for 4 weeks. These co-cultures secrete albumin, synthesize urea, display functional bile canaliculi, and metabolize compounds using active Phase I and Phase II drug metabolism enzymes.

Morphology

Rat_Morphology

Bile Canaliculi

Rat_Bile

Rat HepatoPac co-cultures were created and maintained in vitro for over 4 weeks. No substantial changes to the morphology of micropatterned co-cultures with the maintenance of the polygonal shape.

Liver Function

Rat_Albumin_and_Urea

Albumin and urea secretion of Rat HepatoPac co-cultures over time.

Metabolizing Enzyme Activity

Rat_CYP_profile

When possible, rat specific probe substrates were used. The substrates midazolam, dextromethorphan, diclofenac, buproprion, lauric acid and 50 µM 7-hydroxyl coumarin were incubated for 30 minutes at 37°C with Rat HepatoPac cultures at selected days following co-culture seeding. Using mass spectrometry, cell supernatants were assessed for midazolam hydroxylation by CYP3A, dextromethorphan demethylation by CYP2D, diclofenac hydroxylation by CYP2C, buproprion hydroxylation by CYP2B, lauric acid hydroxylation by CYP4A, and production of 7-OH-coumarin glucuronide and 7-OH-coumarin sulfate.

Product Specifications

HepatoPac kits can be used for any assay that can be run using sandwich, monoculture plates or suspension cultures. Due to the extended in vitro functionality of HepatoPac co-cultures, Hepregen kits enable studies such as low turnover clearance or chronic toxicity studies that could not otherwise be performed. Rat HepatoPac kits are application-specific, containing optimized media and plate layouts.
Applications Include:

  • Toxicology Screening
  • DMPK applications - MetID, Metabolic Stability, Induction, Transporter

Don't see your application? Hepregen scientists are available to discuss your application needs and recommend the appropriate kit, or develop a custom solution.

All kits are provided with pre-patterned primary rat hepatocyte/stromal cell co-cultures. Primary hepatocytes and stromal cells are patterned on Day 0, cultured at Hepregen and shipped to clients on Day 5 of culture. Plates are delivered within 24 to 48 hours of shipping. Cells are ready for dosing on Day 8 of culture (1 to 2 days after receiving the plates). HepatoPac co-cultures remain functional for up to 4 weeks in vitro, providing users with schedule flexibility. We take great pride in the reliability of co-culture manufacturing and their stability during shipping. Learn more about Kit Quality.

 

Documentation

Type Title
Publication Bioactivation and Toxicity of Acetaminophen in a Rat Hepatocyte Micropatterned Coculture System. Journal of Biochemical and Molecular Toxicology. Early Release Epub, August 5, 2013.
Publication Long-Term Stability of Primary Rat Hepatocytes in Micropatterned Co-Cultures. Journal of Biochemical and Molecular Toxicology, first published online January 11, 2013.
Publication Use of Micropatterned Cocultures to Detect Compounds that Cause Drug Induced Liver Injury in HumansToxicological Sciences, Vol. 132, Issue 1, March 2013, Pages 107-117.
Poster Enhancement of Proliferation in a Novel Rat Hepatocyte Co-Culture Model after Mitogenic Stimulation.