Monkey HepatoPac Main

Monkey HepatoPac® Kits

Monkey HepatoPac® kits are an ideal way to conduct all your DMPK, Predictive Toxicology & Mechanistic Toxicology applications. These kits come in a ready-to-use format of monkey HepatoPac co-cultures micropatterned on industry-standard 24- and 96-well plates for streamlined, higher throughput in vitro assays. A variety of monkey kits are available to cover all your application and size needs, and contain everything you need to conduct your assays. Begin dosing as early as 3 days after receiving the kit!

Monkey HepatoPac Main

Kits contain:

  • Monkey HepatoPac Co-Cultures
  • Maintenance Media
  • Application-specific Media and additives
  • Instructions for culture maintenance and application-specific protocols
  • On-site customer training included

Satisfaction Guaranteed! Every kit is backed by Hepregen's performance guarantee and professional customer scientific support. If you are unable to produce desired results in your lab, Hepregen's scientists will run your compounds through our in-house pipeline.

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Validation

Monkey HepatoPac in vitro co-cultures remain viable and highly functional for 4 weeks. These co-cultures secrete albumin, synthesize urea, display functional bile canaliculi, and metabolize compounds using active Phase I and Phase II drug metabolism enzymes.

Morphology

Monkey_Morphology

Monkey HepatoPac co-cultures were created and maintained in vitro for over 4 weeks. No substantial changes to the morphology of micropatterned co-cultures were observed throughout the culture period. Transport of a fluorescent dye via MRP-2 demonstrates the robust network of bile canaliculi at Day 28 of culture.

Liver Function

Monkey_Urea
Monkey HepatoPac co-cultures were created and maintained in culture for 37 days. The rate of urea synthesis was assessed for a period of 37 days for monkey hepatocyte co-cultures. Urea synthesis was observed throughout the culture period demonstrating the extended functionality of in vitro Monkey HepatoPac.

Metabolizing Enzyme Activity

Monkey_Cyp_Profile

Activities of Phase I and Phase II enzyme activities in the Monkey HepatoPac. Human probe substrates were used for these characterization studies. The substrates testosterone, buproprion, dextromethorphan, lauric acid, Promega probe and 50 µM 7-hydroxyl coumarin were incubated for 30 minutes at 37°C with Monkey HepatoPac cultures at selected days following co-culture seeding. Using mass spectrometry, cell supernatants were assessed for testosterone hydroxylation by CYP3A, dextromethorphan demethylation by CYP2D, buproprion hydroxylation by CYP2B, lauric acid hydroxylation by CYP4A, and production of 7-OH-coumarin glucuronide and 7-OH-coumarin sulfate.

Product Specifications

HepatoPac kits can be used for any assay that can be run using sandwich, monoculture plates or suspension cultures. Due to the extended in vitro functionality of HepatoPac™ co-cultures, Hepregen kits enable studies such as low turnover clearance or chronic toxicity studies that could not otherwise be performed. Monkey HepatoPac kits are application-specific, containing optimized media and plate layouts.
Applications Include:

  • Toxicology Screening
  • DMPK applications - MetID, Metabolic Stability, Induction, Transporter

Don't see your application? Hepregen scientists are available to discuss your application needs and recommend the appropriate kit, or develop a custom solution.

All kits are provided with pre-patterned primary rat hepatocyte/stromal cell co-cultures. Primary hepatocytes and stromal cells are patterned on Day 0, cultured at Hepregen and shipped to clients on Day 5 of culture. Plates are delivered within 24 to 48 hours of shipping. Cells are ready for dosing on Day 8 of culture (1 to 2 days after receiving the plates). HepatoPac co-cultures remain functional for up to 4 weeks in vitro, providing users with schedule flexibility. We take great pride in the reliability of co-culture manufacturing and their stability during shipping. Learn more about Kit Quality.

 

Ordering Info

Rat HepatoPac™ kits are application specific and available in 3 sizes.

Part Number Kit Description Breed Application Plate Format # Plates

HPCY-MD-24S

Monkey HepatoPac Met ID Kit - Small Cynomologus Metabolite ID 24-well 1

HPCY-MD-24M

Monkey HepatoPac Met ID Kit - Medium Cynomologus Metabolite ID 24-well 4

HPCY-CL-60S

Monkey HepatoPac Clearance Kit - Small Cynomologus Clearance 60-well 1

HPCY-CL-60M

Monkey HepatoPac Clearance Kit - Medium Cynomologus Clearance 60-well 4

HPCY-TX-96S

Monkey HepatoPac Tox Kit - Small Cynomologus Tox 96-well 1

HPCY-TX-96M

Monkey HepatoPac Tox Kit - Medium Cynomologus Tox 96-well 4

HPCY-TR-24S

Monkey HepatoPac Transporter Kit - Small Cynomologus Transporter 24-well 1

HPCY-TR-24M

Monkey HepatoPac Transporter Kit - Medium Cynomologus Transporter 24-well 4

HPCY-TR-96S

Monkey HepatoPac Transporter Kit - Small Cynomologus Transporter 96-well 1

HPCY-TR-96M

Monkey HepatoPac Transporter Kit - Medium Cynomologus Transporter 96-well 4

HPCY-TR-96L

Monkey HepatoPac Transporter Kit - Large Cynomologus Transporter 96-well 8

Questions about which kit to use? Our team of scientists is happy to assist you in choosing the kit that fits your assay needs.

Documentation

Type Title
Specification Sheet Monkey Donor 1327 Specifications
Poster A Long Term Culture Model for Primary Hepatocytes from Cynomolgus Monkeys