Human HepatoMune® Kits

HepatoMune® is a highly functional in vitro liver platform that models inflammation-mediated hepatotoxicity. Its proprietary patterning of hepatocyte "islands" in a "sea" of stromal and Kupffer cells mimic the physiological microenvironment of the liver, enabling innate response to cytokine-mediated drug-induced liver injury. Its proven longevity of at least 10 days provides the experimental time needed to analyze the inflammatory response and its mechanisms of action.


Tox Kit

Kits Feature:

  • 96-well, HT-ready plate format
  • Proprietary micropatterened tri-cultures of primary hepatocytes, fibroblasts and primary Kupffer cells
  • 10 day culture longevity
  • Data predictive of human inflammation-mediated toxicity

Kits contain:

  • Human HepatoMune® Co-Cultures
  • Kupffer Cells
  • Maintenance Medium for both pre and post Kupffer cell augmentation
  • Application-specific Media and Additives
  • Instructions for Culture Maintenance and Application-specific Protocols
  • On-site Customer Training Included

Satisfaction Guaranteed! Every kit is backed by Hepregen's performance guarantee and professional customer scientific support. If you are unable to produce desired results in your lab, Hepregen's scientists will run your compounds through our in-house pipeline.

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Validation

Human HepatoMune® in vitro tri-cultures remain viable and highly functional for 10 days. These tri-cultures secrete albumin, synthesize urea, display functional bile canaliculi, and metabolize compounds using active Phase I and Phase II drug metabolism enzymes. The presence of functional Kupffer cells in HepatoMune tri-cultures is confirmed via pHrodo-S. aureus phagocytosis and CD68 staining. Primary hepatocyte function remains unaffected by the presence of activated Kupffer cells.

Functional Kupffer Cells

Functional Kupffer Cells
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HepatoMune tri-culture were created using patented microfabrication tools and consist of primary hepatocytes arranged in optimized domains and surrounded by stromal fibroblasts. Cryopreserved primary Kupffer cells are added on day 7 of culture. pHrodo and CD68 staining illustrates the presence of functional Kupffer cells on day 10 of culture.

Functional Hepatocytes

Functional Kupffer Cells
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Augmentation with primary human Kupffer cells does not significantly affect functionality of the primary hepatocytes as determined by Urea synthesis or Cyp3A4 activity.


Cytokine-mediated CYP3A4 Downregulation

Cytokine-mediated CYP3A4 Downregulation
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Kupffer cell augmentation does not alter hepatocyte viability, as measured by ATP levels, following cytokine exposure over a 4 day dosing cycle. Addition of IL-6 suppresses CYP3A4 activity in HepatoMune, while addition of IL-1β to HepatoMune produces a Kupffer cell-dependent decrease in CYP3A4 activity.

Product Specifications

HepatoMune® kits can be used for any toxicology assay that can be run using sandwich, monoculture plates or suspension cultures. Due to the extended in vitro functionality of HepatoMune® tri-cultures, Hepregen kits enable extended studies of inflammation-mediated hepatotoxicity.

 

To engineer HepatoMune, hepatocytes are organized into colonies of prescribed, empirically-optimized dimensions using microfabrication tools and subsequently surrounded by supportive non-parenchymal cells. This combination is then augmented with primary, species-matched Kupffer cells at a precise hepatocyte:Kupffer cell ratio of 1:0.4. The presence of functional Kupffer cells in HepatoMune tri-cultures is confirmed via pHrodo-S. aureus phagocytosis and CD68 staining. The resulting HepatoMune tri-culture represents a liver microenvironment that mimics an inflamed liver state.

HepatoMune® tri-cultures remain functional for at least 10 days in vitro. We take great pride in the reliability of co-culture manufacturing and their stability during shipping. Learn more about Kit Quality.

 

Ordering Info

Human HepatoMune® kits are application specific and available in 2 sizes. Multiple human hepatocyte lots are available. Please contact your local sales manager for specific lot information.

Part Number Kit Description Application Plate Format # Plates
HMHU-TX-96S Human HepatoMune® Tox Kit - Small Tox Tox 96-well 1
HMHU-TX-96M Human HepatoMune® Tox Kit - Medium Tox Tox 96-well 4

Questions about which kit to use? Our team of scientists is happy to assist you in choosing the kit that fits your assay needs.

Documentation

Type Title
Specification Sheet Human Donor 0002 Specifications
Specification Sheet Human Donor 3121A Specifications
Application Note HepatoMune: A Bioengineered Micro-liver Platform for the Study of Inflammatory Responses in Toxicology Investigation