If you’ve been following the Hepregen blog and reading up on Hepregen’s products, HepatoPac® and HepatoMune™, you may be aware of the fact that we often point out the value of the unprecedented and unmatched longevity and stability of functionality exhibited by our micropatterned, co-culture platform. This extended in vitro functionality (stable cytochrome P450 and Phase II enzymatic activity, albumin & urea production, transporter function enables investigation of the metabolism and transport of low turnover compounds and of chronic toxicity that simply cannot be performed in traditional short-lived culture systems, such as S9 fractions, microsomes, hepatic cell lines, or even primary hepatocytes that are not in a co-cultured, micropatterned configuration.
It is also noteworthy that the optimal metabolic activity that characterizes Hepregen’s micropatterned platform makes it the ideal liver model for obtaining the most accurate data available for short-term evaluations of drug metabolism or for other screening applications. HepatoPac outperforms traditional models, especially in their capacity to resolve major, circulating metabolites found in vivo. This blog is intended to highlight just a few examples of the functional features of HepatoPac. These will include the ability to,
o Generate major, in vivo circulating and excretory metabolites both early (hours) and late (days) post compound exposure
o Resolve time-dependent (early and late) appearance of in vivo relevant metabolites across human and pre-clinical species (monkey, dog, rat) within a single experiment
o Better predict in vivo hepatic clearance rates for high-, intermediate-, and low-turnover compounds